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Wednesday, March 12, 2014

Thursday, May 3, 2012

Gel filtration/Size-exclusion Chromatography

Principles of  Gel filtration chromatography


Gel filtration, also called size-exclusion chromatography, separates proteins according to their size. There are pores in the gel filtration matrix and these pores permit the buffer and smaller proteins to enter into it but reject larger proteins and protein complexes. Therefore, larger proteins move around the matrix particles and elute from the column first before smaller proteins. 

Tuesday, May 1, 2012

Textbooks available on the Internet


Biochemistry, 5th edition
Jeremy M Berg, John L Tymoczko, and Lubert Stryer.

Genomes, 2nd edition
Terence A Brown.


Immunobiology, 5th edition
The Immune System in Health and Disease
Charles A Janeway, Jr, Paul Travers, Mark Walport, and Mark J Shlomchik.

Bruce Alberts, Alexander Johnson, Julian Lewis, Martin Raff, Keith Roberts, and Peter Walter.

Harvey Lodish, Arnold Berk, S Lawrence Zipursky, Paul Matsudaira, David Baltimore, and James Darnell.


More Textbooks

Sunday, April 29, 2012

Nutrients and Their Functions

Carbohydrate:

Carbohydrates (sugar and starches) are the most efficient sources of energy and are known as the “fuel of life.”  They are abundantly found in most plant food sources. Complex  carbohydrates  (starches)  are  in breads,  cereals,  pasta,  rice,  dry  beans  and  peas,  and other vegetables, such as potatoes and corn. Simple carbohydrates are found in sugars, honey, syrup, jam, and many desserts. 

Thursday, April 26, 2012

Vitamins and Minerals: Their presence in adult DRI


The Dietary Reference Intakes (DRIs) are a set of scientifically based nutrient reference values for healthy populations. Vitamins and minerals are nutrients from our environment that we need for all the essential reactions in our cells to occur. Our bodies can make small amounts of certain vitamins, but we cannot manufacture minerals. Every mineral in our body must first come from outside our