Gel filtration/Size-exclusion Chromatography

Principles of  Gel filtration chromatography

Gel filtration, also called size-exclusion chromatography, separates proteins according to their size. There are pores in the gel filtration matrix and these pores permit the buffer and smaller proteins to enter into it but reject larger proteins and protein complexes. Therefore, larger proteins move around the matrix particles and elute from the column first before smaller proteins. 

Textbooks available on the Internet

Biochemistry, 5th edition Jeremy M Berg, John L Tymoczko, and Lubert Stryer.

Genomes, 2nd edition Terence A Brown.

Immunobiology, 5th edition The Immune System in Health and Disease Charles A Janeway, Jr, Paul Travers, Mark Walport, and Mark J Shlomchik.

Molecular Biology of the Cell, 4th edition Bruce Alberts, Alexander Johnson, Julian Lewis, Martin Raff, Keith Roberts, and Peter Walter.

Molecular Cell Biology, 4th edition Harvey Lodish, Arnold Berk, S Lawrence Zipursky, Paul Matsudaira, David Baltimore, and James Darnell.

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