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Wednesday, March 12, 2014
Thursday, May 3, 2012
Gel filtration/Size-exclusion Chromatography
Principles of Gel filtration chromatography
Gel filtration, also called size-exclusion chromatography, separates proteins according to their size. There are
pores in the gel filtration matrix and these pores permit the buffer and
smaller proteins to enter into it but reject larger proteins and protein
complexes. Therefore, larger proteins move around the matrix particles and
elute from the column first before smaller proteins.
Tuesday, May 1, 2012
Textbooks available on the Internet
Biochemistry, 5th edition
Jeremy M Berg, John L Tymoczko, and Lubert Stryer.
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Genomes, 2nd edition
Terence A Brown.
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Immunobiology, 5th edition
The Immune System in Health and Disease
Charles A Janeway, Jr, Paul Travers, Mark Walport, and Mark J
Shlomchik.
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Molecular Biology of the Cell, 4th edition
Bruce Alberts, Alexander Johnson, Julian Lewis, Martin Raff, Keith
Roberts, and Peter Walter.
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Molecular Cell Biology, 4th edition
Harvey Lodish, Arnold Berk, S Lawrence Zipursky, Paul Matsudaira,
David Baltimore, and James Darnell.
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More Textbooks
Sunday, April 29, 2012
Nutrients and Their Functions
Carbohydrate:
Carbohydrates (sugar and starches) are the most efficient sources of energy and are known as the “fuel of life.” They are abundantly found in most plant food sources. Complex carbohydrates (starches) are in breads, cereals, pasta, rice, dry beans and peas, and other vegetables, such as potatoes and corn. Simple carbohydrates are found in sugars, honey, syrup, jam, and many desserts.
Thursday, April 26, 2012
Vitamins and Minerals: Their presence in adult DRI
The Dietary Reference Intakes (DRIs) are a set of
scientifically based nutrient reference values for healthy populations. Vitamins and minerals
are nutrients from our environment that we need for all the essential reactions
in our cells to occur. Our bodies can make small amounts of certain vitamins,
but we cannot manufacture minerals. Every mineral in our body must first come
from outside our
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