Principles of Gel filtration chromatography
Gel filtration, also called size-exclusion chromatography, separates proteins according to their size. There are
pores in the gel filtration matrix and these pores permit the buffer and
smaller proteins to enter into it but reject larger proteins and protein
complexes. Therefore, larger proteins move around the matrix particles and
elute from the column first before smaller proteins.
Among the proteins applied
onto the column, the largest proteins come out from the
column first since they cannot enter into the pores. Unlike the largest
proteins, medium-sized proteins can enter the larger size pores in the matrix,
and so they arrive at the end of the column later. On the other hand, small
proteins are able to enter all the pores. So, they have the largest volume to
pass through before emerging from the column last. And thus, proteins are
separated according to their size.
No comments:
Post a Comment