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Sunday, April 29, 2012
Thursday, April 26, 2012
Vitamins and Minerals: Their presence in adult DRI
The Dietary Reference Intakes (DRIs) are a set of
scientifically based nutrient reference values for healthy populations. Vitamins and minerals
are nutrients from our environment that we need for all the essential reactions
in our cells to occur. Our bodies can make small amounts of certain vitamins,
but we cannot manufacture minerals. Every mineral in our body must first come
from outside our
Tuesday, April 24, 2012
DNA Cloning/ Recombinant DNA Technology/ Genetic Engineering
A clone is an identical copy. This term originally applied to cells of a
single type, isolated and allowed to reproduce to create a population of
identical cells. DNA cloning involves separating a specific gene or DNA segment from a larger
chromosome, attaching it to a small molecule of carrier DNA, and then
replicating this modified DNA thousands or millions of times through both an
Carbohydrate digestion begin in the mouth
Salivary gland produces saliva,
which digests carbohydrate in mouth. This early step of digestion facilitates
the absorption of glucose and thus facilitates getting energy from carbohydrate than
other foodstuffs like protein, fat etc.
What does the thyroid gland do?
The thyroid gland controls how quickly the body
uses energy, makes proteins, and controls how sensitive the body is to other
hormones. It participates in these processes by producing thyroid hormones, the
principal ones being triiodothyronine (T3) and thyroxine which can sometimes be
referred to as tetraiodothyronine (T4). These hormones regulate the rate of
metabolism and affect the growth and rate of function of many other systems in
the body. T3 and T4 are synthesized from both iodine and tyrosine. The thyroid
also produces calcitonin, which plays a role in calcium homeostasis.
Monday, April 23, 2012
General Transcription Factors
General
transcription factors (GTF's) or basal transcription factors are protein
transcription factors that have been shown to be important in the transcription
of class II genes to mRNA templates. Many of them are involved in the formation
of a preinitiation complex, which, together with RNA polymerase II, bind to and
read the single-stranded DNA gene template.
Why and how does salts precipitate proteins?
Salt dehydrates water around the
protein molecules dissolved in solution. As a result, the solubility of protein
molecules is reduced. This process brings the protein molecules close to
interact each other. And thus the protein molecules settle down as precipitate.
Vitamins and minerals work together to maintain bone health
Vitamins and minerals have
different functions in the body. Sometimes they may work together at times.
Vitamins and minerals are needed to maintain the health of tissue, organs,
muscles, bones and blood. Vinatim D is essential to absorb calcium. Actually, without
vitamin D, the body can't form a hormone called calcitriol, which will
contribute to insufficient dietary calcium absorption. If the body doesn't get
enough calcium, it will extract it from the bones. This may cause Osteoporosis.
Classification of Nutrients
Vitamins and Minerals: Their presence in adult DRI
Related Post
Nutrition: Some DefinitionsClassification of Nutrients
Vitamins and Minerals: Their presence in adult DRI
Principles of DEAE cellulose column method
Separation
and purification of proteins using ion exchange chromatography is based
primarily on differences in the ionic properties of surface of amino acid.
Proteins bind to ion exchangers by electrostatic forces between the protein’s
surface charges (mainly) and the dense clusters of charged groups on the
exchangers. The charges are of course balanced by counterions such as metal
ions, chloride ions, and sometimes buffer ions. A protein must displace the
counterions and
Difference between Prokaryote and Eukaryote: Explanation
The
differences between prokaryote and eukaryote range from size to intracellular
movement. There are differences in many important points in-between. Unlike
prokaryotic cells, typical eukaryotic cells are much larger with a thousand to
a million times larger cell volumes than those of bacteria. The distinguishing
characteristics of eukaryotes are the nucleus, which has a complex internal
Actin: Roles in Cell Division
Actin is a major component of the
cytoarchitecture and plays significant roles in cell migration and cell
division. It accomplishes its function over cell division in association with
myosin. The most dramatic example of actin-myosin
contraction in nonmuscle cells is provided by Cytokinesis—the division of a
cell into two following mitosis. Toward the end of mitosis in animal cells, a contractile ring
consisting of actin filaments and myosin
II assembles just underneath the plasma membrane.
Its contraction pulls the plasma membrane progressively inward, constricting
the center of the cell and
PCR
Polymerase
chain reaction (PCR) is a repetitive procedure that results in geometric
amplification of a specific DNA sequence. In this method, two synthetic
oligonucleotides are prepared, complementary to sequences on opposite strands
of the target DNA at positions just beyond the ends of the segment to be
amplified. The oligonucleotides serve as replication primers that can be
extended by
Cell Cycle
Cell cycle is the period between the
formation of a cell by division of its mother cell and the time when the cell
itself divides to form two daughter cells. Cell division in eukaryotes occurs in four well-defined stages. In
the S (synthesis) phase, the DNA is replicated to produce copies for both
daughter cells. In the G2
phase (G indicates the gap between divisions), new proteins are synthesized and
the cell approximately doubles in size. In the M phase (mitosis), the maternal
Sunday, April 22, 2012
What is the relevance of having two membranes in the mitochondria?
This is required to maintain the electrochemical
potential in the space between two membranes. This electrochemical potential
represents proton-motive force which is ultimately used to generate ATP by ATP
synthase situated in the inner membrane.
What does the abstract mean in a scientific report?
It is a summary of scientific work. You may
write 200-250 words to describe your work. Description may contain why the
research has taken, how it has done, what things have considered and what are
the major findings. The sum up of all these may construct an abstract of a
scientific work
What's the main energy transfer during respiration?
ATP is formed due to transfer of electrons
through respiratory chain located in the inner membrane of mitochondria. Actually,
electrochemical gradient is formed in the intramembrane space simultaneously
with electron transfer. And proton-motive force is subsequently is used to
drive the synthesis of ATP. So, in human respiration the chemical energy is
converted to heat and electric energy.
Salting Out Technique: Purpose
Salting out is a technique that has been using
to separate desired substance from a solution. Adding of an appropriate percentage of salt
to a solution causes the water molecules to be exhausted gradually from the surrounding of the desired
molecules that are polar in the water. As a result, the desired
substances/molecules come close together and are settled down.
It may be applicable to enzymes, proteins etc.
Prokaryotes and Eukaryotes: Difference
There are many parameters. Nonetheless,
prokaryotic genome is nucleoid, not surrounded by memebrane and DNA is
associated with nonhistone proteins. Conversely, DNA is complexed
with histone and nonhistone proteins in eukaryotic chromosome and chromosome is in nucleolus, which has membranous envelope.
No membrane-bound organelles, mitochondrion and cytoskeleton are available in prokaryotic. On the other hand, eukaryote has many membrane-bound organelles, possesses oxidative enzymes and has complex cytoskeleton.
No membrane-bound organelles, mitochondrion and cytoskeleton are available in prokaryotic. On the other hand, eukaryote has many membrane-bound organelles, possesses oxidative enzymes and has complex cytoskeleton.
Heart and Circulatory System: Problems
The circulatory system is composed of the heart and blood vessels,
including arteries, veins, and capillaries. Our bodies actually have two
circulatory systems: The pulmonary circulation is a short loop from the heart
to the lungs and back again, and the systemic circulation (the system we usually
think of as our circulatory system) sends blood from the heart to all the other parts of our bodies and back again.
Problems of the Heart and Circulatory System
Heart and circulatory problems are grouped into two categories:
congenital (problems present at birth) and acquired (problems developed some
time after birth).
Congenital heart defects
These
abnormalities in the heart's structure are present at birth and these defects
occur while the fetus is developing in the mother's uterus and it's not usually
known why they occur. Some congenital heart defects are caused by genetic
disorders, but most are not. What all congenital heart defects have in common,
however, is that they involve abnormal or incomplete development of the heart.
A common
sign of a congenital heart defect is a heart murmur — an abnormal sound (like a blowing or whooshing
sound) that's heard when listening to the heart. Usually a heart murmur is
detected by a doctor who's listening to the heart with a stethoscope during a
routine exam. Murmurs are very common in children and can be caused by
congenital heart defects or other heart conditions.
Biochem Blog and Info: Retrovirus and Bacteriophage
Biochem Blog and Info: Retrovirus and Bacteriophage: A retrovirus is an RNA virus that is duplicated in a host cell using the reverse transcriptase enzyme to produce DNA from its RNA g...
Synthases and Synthetases; Ligases and Lyases; Kinases, Phosphatases, and Phosphorylases
Synthases catalyze condensation
reactions in which no nucleoside triphosphate (ATP, GTP, and so forth) is
required as an energy source. e.g. Citrate synthase
Synthetases catalyze
condensations that do use
ATP or another nucleoside triphosphate as a source of energy for the synthetic
reaction. Succinyl-CoA synthetase is such an enzyme.
Ligases are enzymes that
catalyze condensation reactions in which two atoms are joined using ATP or
another energy source. (Thus synthetases are ligases.) DNA ligase, for example,
closes breaks in DNA molecules, using energy supplied by either ATP or NAD+; it is widely used
in joining DNA pieces for genetic engineering.
Saturday, April 21, 2012
Classification of Nutrients
According to their chemical nature
Nutrients are organic and
inorganic compounds in foods and, according to their chemical nature, are
classified into the following types of substances:
Protein
Carbohydrates
Lipids
Vitamins
Mineral salts
Water
Nutrients are normally
obtained by the ingestion of foods. Organic nutrients include carbohydrates,
proteins or amino acids, lipids, and vitamins. Inorganic nutrients include
minerals. Water is sometimes included in a listing of nutrients
Nutritional Classification of Foods
The different groups of foods may be
broadly classified under three heads from the nutritional point of view.
Energy-yielding
foods;
Body-
building foods and
Protective
foods.
Energy Yielding Foods
The group includes
foods rich in carbohydrates and also fats. They may be broadly divided into two
groups:
Cereals,
roots and tubers and
Carbohydrates
and Fats.
Cereals
provide, in addition to energy the greater part of the proteins, certain
minerals and vitamins in the deits of the low income groups in the developing
countries. Roots and tubers also provide some amounts of proteins, minerals and
vitamins while pure carbohydrates and fats provide only energy.
Body Building Foods
Foods rich in
proteins are called body building foods. These may be broadly divided into two
groups:
Milk,
egg and fish rich in proteins of high biological value and
Pulses,
oilseeds and nuts and low fat oilseed flours rich in proteins of medium
nutritive value.
Protective Foods
Foods rich in proteins, vitamins and minerals are termed protective foods. Protective foods are broadly classified into two groups:
Foods
rich in vitamins, minerals and proteins of high biological value e.g.., milk,
egg, fish and liver and
Foods
rich in certain vitamins and minerals only e.g., green leafy vegetables and
some fruits.
Depending on the importance
In terms of participation in metabolic reactions of the
organism as a whole, the nutrients can be:
Nonessential
Nutrients:
Nonessential Nutrients which are not vital to the body and that, under certain conditions,
are synthesized via precursor molecules (usually essential nutrients).
Therefore, the body does not need regular intake of such a condition to obtain
the precursors of their environment. These are produced by the body's
metabolism.
Essential
nutrients:
Essential nutrients are those that are vital to the agency as you can not
synthesize. That is, are substances that are so indispensable to obtain the
environment. For humans, these include essential fatty acids, essential amino
acids, some vitamins and certain minerals. Oxygen and water are also essential
for human survival, but usually not considered as nutrients when consumed in
isolation.
Humans can get energy from a variety of fats, carbohydrates, proteins and ethanol and other compounds can be synthesized (eg, certain amino acids) from essential nutrients.
The nutrients have a significant role on health, whether beneficial or toxic. For example, sodium is a nutrient that is involved in water balance processes when provided in adequate amounts. But his excessive intake in the diet can promote hypertension.
Humans can get energy from a variety of fats, carbohydrates, proteins and ethanol and other compounds can be synthesized (eg, certain amino acids) from essential nutrients.
The nutrients have a significant role on health, whether beneficial or toxic. For example, sodium is a nutrient that is involved in water balance processes when provided in adequate amounts. But his excessive intake in the diet can promote hypertension.
According to its mass
Depending on the quantity necessary for cells and organisms are classified as:
Macronutrients
are required in large quantities daily (usually in the order of grams). These
nutrients are involved as substrates in metabolic processes for energy. These include
proteins, carbohydrates and fats and are the basis of any diet.
Related Post
Nutrition: Some Definitions
Vitamins and Minerals: Their presence in adult DRI
Nutrients and Their Functions
Friday, April 20, 2012
Nutrition: Some Definitions
Food: Food
is the prime necessity of life. The food we eat is digested and assimilated in
the body and used for its maintenance and growth. Food also provides energy for
doing work. So,
food is that which nourishes the body.
food is that which nourishes the body.
Nutrients:
Nutrients
are those organic and inorganic compounds that a living organism must acquire
from the environment to support essential life processes, including 1basal
metabolism, growth and maintenance of body tissues, activity, reproduction, and
maintenance of general health.
Saturday, April 14, 2012
Biochemistry: Has a wide range of applications
Biochemistry is a hub of
life science. This knowledge of biochemistry has been applying to medicine,
dentistry, and veterinary medicine since long. In food science, this branch of
biology is involved in the development of abundant and cheap sources of
nutritious foods, determination of the chemical composition of foods and development
of methods to extract nutrients from waste products. Biochemistry is also engaged in the invention
the ways to prolong the shelf life food products.
Biochemistry and Agriculture
Agricultural biochemistry is not only the chemistry of
living things; it's a part and parcel of modern life science. It is a combination
of mathematics, chemistry, physics, biochemistry, genetics, molecular cell
biology, and research. In agriculture, biochemists involve in the study of
interaction between herbicides and plants. They explore the structure-activity
relationships of compounds, determine their ability to inhibit growth, and
evaluate the toxicological effects on surrounding life.
Living Matter: Identifying Characteristics
There are some unique characteristics that make difference the
living organism from non-living world. The distinguishing features of living
organisms are as follows:
1) High level of chemical complexity and minute organization
Living organism is very complex. Hundreds of thousands
of different molecules make up a cell’s complicated internal structures. Distinguishing
sequence of subunits, unique three-dimensional
structure, and its highly specific selection of binding partners in the cell
are some features of every molecule.
Saturday, April 7, 2012
Biochemistry and Medicine
Two fields of life science-biochemistry and medicine are closely
related. Understanding and maintenance of health and the understanding and effective
treatment of diseases are the two major concerns for workers involved in health
sciences. Biochemistry has much impact on both of these fundamental concerns of
medicine. Actually, a broad interrelationship exists between biochemistry and
medicine. Biochemical studies have lighted up many aspects of health and disease.
In other words, the study of various aspects of health and
disease has opened up new areas of biochemistry.
Tuesday, April 3, 2012
Biochemistry and Nutrition
Many disciplines have
emerged from Biochemistry . Nutritional biochemistry is a branch of biochemistry
and is made up of the core knowledge, concepts, and methodology related to
the chemical properties of nutrients and other dietary constituents and to
their biochemical, metabolic, physiological, and epigenetic
functions. This discipline has influence on mammalian physiology, health,
and behavior.
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